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mini-courses
Transcriptome analysis: the theory behind and analysis on the galaxy platform
Master's student at the Department of Biochemistry and Immunology (UFMG).
Date: May 18, from 08:00 to 12:00 and from 14:00 to 18:00, online, 30 spots.
The mini-course aims to address through a short introduction: the transcriptome, the difference between a transcriptome and a genome, the transcription process and the regulation of gene expression, alignment and pseudo-alignment, construction of cDNA libraries, the production of sequences cDNA partials, transcript detection methodologies, study of differential expression of transcripts, bioinformatics in the study of transcriptome and gene annotation. In the practical part of the course, the most used programming languages will be addressed, what is necessary to analyze a transcriptome, how to perform an alignment using the Galaxy online tool, the alignment result and the differential expression result.
The essentials of genetics: activity guide for high school
Speakers: Lorrayne Evangelista de Sousa and Rafaella Cardoso Ribeiro
Rafaella Cardoso: postdoctoral fellow in Genetics (UFMG).
Lorrayne Evangelista de Sousa: Master in Genetics (UFMG).
Date: May 14, from 08:00 to 12:00, In-person (Neducom), 20 vacancies.
Lorrayne
Mendelism; Crossing and Probabilities; Heredity; Mitosis and Meiosis. Genetics of the ABO System; Transcription, Translation and Allelic Interactions.
Rafaella
Phylogenetics for Functional Prediction of Genes and Proteins
Lecturer: Laila Alves Nahum, Elvira Cynthia Alves Horácio and Millena Ferreira Fernandes
Graduate Program in Genetics (UFMG).
Date: 16th to 27th of May, online, 20 spots.
Phylogenetics studies the relationships between taxa and/or macromolecules over evolutionary time. This transdisciplinary approach has applications in health, environment, society, etc. The main objective of this mini-course is to discuss concepts of phylogenetics and molecular evolution for the functional prediction of genes and proteins not yet characterized experimentally. Examples of functional prediction in some gene and protein families in different taxa will be discussed. Together, the works contribute to several areas of knowledge. Teaching Methodology: Study of the previously indicated bibliography and discussion forum during online classes. Assessment Methodology: Active participation in discussions and seminar presentations by students. Bibliographic References: chapters of books and scientific articles indicated and made available before the classes.
Theoretical-practical course on protein structure prediction
Minister: Viviane Correa Santos
Master's student at the Department of Biochemistry and Immunology (UFMG).
Date: 24th, 25th, 26th and 27th of May. From 5:00 pm to 8:00 pm, online and in person, 20 spots.
1- Structure and organization of proteins;
2 - Reasons to use the Comparative Modeling technique;
3 - Introduction to the technique of Comparative Modeling;
4 - Introduction to Modeller and PyMOL software;
5 - Introduction to other protein structure prediction techniques;
6 - Model evaluation;
7 - Tutorial for predicting the tertiary structure of proteins with Modeller.
Protein analysis using the Western Blotting technique
Lecturer: Ana Carolina Valente Santos Cruz de Araujo
Postgraduate Program in Genetics (UFMG).
Date: 30th and 31st of May, from 10:00 to 12:00, online, 20 spots.
The course "Analysis of proteins using the Western Blotting technique" will address the workflow of this technique, with a conceptual and practical approach, which includes: polyacrylamide gel electrophoresis (SDS-PAGE), transfer of proteins to the membrane, blocking of non-specific sites, incubation with antibodies, membrane development and analysis of images by densitometry. In addition, applications of the technique and practical details essential for good resolution of the results will be addressed.
Gene cloning, heterologous expression of proteins and purification of biotechnological products
Lecturer: Fabio Mambelli Silva
Department of Biochemistry (UFMG).
Date: June, online, 20 spots.
Class 1, synchronous: The principles of genetic engineering aimed at cloning genes of biotechnological interest will be discussed. Cloning methods and vectors, cloning and expression lines, protocols and challenges in the expression of recombinant proteins (recovered in soluble and insoluble form) in Escherichia coli system will be discussed. An activity to be performed asynchronously during the week will be passed.
Class 2, synchronous: The activity will be evaluated and then chromatographic techniques will be discussed for the purification of bioproducts in an active, functional form and with a high degree of purity.
Epigenetic changes in disease development
Minister: Juliana de Oliveira Cruz
Department of Genetics (UFMG).
Date: June 06th and 07th, from 09:00 to 12:00, online, 50 spots.
Epigenetic mechanisms are involved in the entire process of development, differentiation and cellular determination of an organism. Epigenetics is everything that is beyond the DNA sequence, participating in the regulation of gene transcription/translation. Among the known epigenetic mechanisms are: DNA methylation, post-translational modifications of histone proteins, methylation of RNAs and non-coding RNAs. Of these, DNA methylation is the most studied and best understood, acting from health to disease. Complex and multifactorial diseases such as cancer and preeclampsia develop through different factors and biological pathways, thus having the participation of DNA methylation involved in these processes. Thus, we will have two theoretical/practical classes: 1: introduction to epigenetic modifications; 2: Unraveling DNA methylation and disease development.
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